Downregulation of CYP39A1 Serves as a Novel Biomarker in Hepatocellular Carcinoma with Worse Clinical Outcome

Figure 7

The relationship between CYP39A1 and the cell growth of HCC. (a) Relative CYP39A1 mRNA expression in the EV and OE groups after infection with CYP39A1 lentiviral plasmid in HepG2 and SMMC-7721 cells; (b) cell viability was inhibited after CYP39A1 was overexpressed in HepG2 and SMMC-7721 cells; (c) CERES dependency score of CYP39A1 in 22 HCC cell lines in the CRISPR combined database; (d) and (e) the relative protein expression of CYP39A1, p-NF-κB, Keap1, and Nrf2 after infection with CYP39A1 lentiviral plasmid in HepG2 and SMMC-7721 cells; histograms indicated the relative expression levels of CYP39A1, p-NF-κB, Keap1, and Nrf2. Data were expressed as the ; . , , versus the EV group; (f) the protein expression of CYP39A1, p-NF-κB, Keap1, and Nrf2 in AKT/c-Met-induced HCC mouse models were determined by Western blot analysis; histograms indicated the relative expression levels of CYP39A1, p-NF-κB, Keap1, and Nrf2. Data were expressed as the ; . ^#, ^##, ^### versus the WT group; , , versus the AKT/c-Met group.

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