The Synergistic Reducing Drug Resistance Effect of Cisplatin and Ursolic Acid on Osteosarcoma through a Multistep Mechanism Involving Ferritinophagy

Figure 1

Synergistic antitumour effect of UA and Cis on OS cells in vitro. HOS and 143B cells were treated with different concentrations of UA and Cis for 24 h, and cell viability was assessed by the CCK-8 assay (a) (). The CCK-8 assay was used to evaluate cell viability under UA and/or Cis treatment (b) (). The colony formation ability of HOS and 143B cells was assessed after monotherapy or treatment with the combination of UA and Cis for 14 days (c) (). HOS and 143B cells were treated with UA and/or Cis for 24 h, and the apoptosis rate was measured by flow cytometry (d) (). HOS and 143B cells were pretreated with UA and/or Cis for 24 h and subjected to calcein-AM/PI staining (e) (, scale bar: 200 μm; calcein-AM: live cells; PI: dead cells). The data are presented as the . vs. the control group.

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