Melatonin Exerts Cardioprotective Effects by Inhibiting NLRP3 Inflammasome-Induced Pyroptosis in Mice following Myocardial Infarction

Figure 2

Effect of melatonin on NLRP3 inflammasome-mediated cardiac pyroptosis in MI mice. (a) Representative images from TUNEL staining in different groups. Scale bar, 20 μm. (b) Melatonin treatment decreased the release of LDH in MI mice. vs. sham, ^# vs. MI. . (c) The level of GSDMD-N was decreased by melatonin treatment. vs. sham, ^# vs. MI. . (d, e) Melatonin treatment reduced the levels of NLRP3 and cl.casp-1 in the hearts of MI mice. vs. sham, ^# vs. MI. . (f) The mRNA levels of NLRP3 and caspase-1 in the different groups were detected by real-time PCR. vs. sham, ^# vs. MI. . (g, h) The levels of IL-1β and IL-18 in heart tissue from different groups. vs. sham, ^# vs MI. . MI: myocardial infarction; cl.casp-1: cleaved caspase-1; MLT: melatonin.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)