Melatonin Exerts Cardioprotective Effects by Inhibiting NLRP3 Inflammasome-Induced Pyroptosis in Mice following Myocardial Infarction

Figure 5

Effect of melatonin on NLRP3 inflammasome-mediated pyroptosis through inhibition of the TLR4/NF-κB signaling pathway. (a) LPS (1 μg/mL) blocked the effect of melatonin on H9C2 cardiomyocyte cell death. Live cells were stained with calcein-AM (green), and dead cells were stained with EthD-III (red). Scale bar, 40 μm. (b–f) Representative images and statistical analysis of GSDMD-N (b), NLRP3 (c), and cl.casp-1 (d), mature IL-1β (e), and mature IL-18 (f) data from different groups. vs. ctl, ^# vs. hypoxia, ^$ vs. MLT. . MLT: melatonin.

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