Inhibition of PTEN Ameliorates Secondary Hippocampal Injury and Cognitive Deficits after Intracerebral Hemorrhage: Involvement of AKT/FoxO3a/ATG-Mediated Autophagy

Figure 2

PTEN deletion improved ICH-induced cognitive deficits. (a, b) Silencing efficiency of PTEN was detected by real-time PCR and Western blot analysis at 72 h after infection. (c) Representative images showed swim paths on days 12 after ICH. (d–g) Escape latency (d), target quadrant time (e), times across the platform (f), and velocity (g) were analyzed in the MWM test at 8-13 d postinjury. (h, i) Spatial working memory was assessed using the Y-maze test by monitoring spontaneous alteration at 7 d postinjury. (j, k) Latency to reenter the dark compartment (error latency) and error times were evaluated in the PAT test at 14 d post-ICH. The data are represented as the of five independent experiments. compared with the Sham group; compared with the ICH group.

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