Pegylated Recombinant Human Arginase 1 Induces Autophagy and Apoptosis via the ROS-Activated AKT/mTOR Pathway in Bladder Cancer Cells

Figure 1

Sensitivity of bladder cancer cells to BCT-100 correlated with the expression of ASS1 and OTC. (a) Cell growth inhibition evaluated by MTT assay in bladder cancer cell lines (J82, SCaBER, T24, and 5637), human bladder cell line (SV-HUC-1), and A549 cells treated with BCT-100 for 72 h. (b) Basal expression levels of ASS1 and OTC in bladder cancer cells evaluated by Western blot. (c) Cell survival rate of ASS1-silenced 5637 cells (72 h) evaluated by MTT assay. (d) Expression of C-PARP and ASS1 in ASS1-silenced 5637 cells treated with BCT-100 for 72 h. β-Actin was used as a loading control.

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