Pegylated Recombinant Human Arginase 1 Induces Autophagy and Apoptosis via the ROS-Activated AKT/mTOR Pathway in Bladder Cancer Cells

Figure 2

BCT-100 induced cytotoxicity and lowered the level of arginine in bladder cancer cells. (a, c) Intracellular PEG, Caspase 3, Survivin, and C-PARP evaluated by Western blot in T24 cells exposed to BTC-100 (20 mU/ml) for 72 h. (b) Intracellular arginine levels following BCT-100 treatment for 72 h. (d) Apoptotic rates determined by Annexin V and PI staining analyzed by flow cytometry in cells treated with BCT-100 (20 mU/ml) for 72 h. (e) TUNEL assay indicating the presence of apoptotic cells exposed to BCT-100 (20 mU/ml) for 72 h. Data are represented as the of three independent experiments., , and , as evaluated by Student’s -test.

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