Pegylated Recombinant Human Arginase 1 Induces Autophagy and Apoptosis via the ROS-Activated AKT/mTOR Pathway in Bladder Cancer Cells

Figure 3

Apoptosis induced by BCT-100 was mitochondrial-dependent and accompanied by ROS production. (a) Elevation of H₂O₂ after BCT-100 treatment (20 mU/ml) for 24 h analyzed by flow cytometry. (b) Mitochondrial membrane depolarization shown by JC-1 staining in T24 cells upon BCT-100 (20 mU/ml) exposure for 24 h. (c, d) Subcellular localization of Cyt-c and Smac assessed by Western blot in cells treated with BCT-100 (20 mU/ml) for 72 h. COX IV and β-actin were used as loading controls in mitochondrial and cytosolic fraction, respectively. Data are represented as the of three independent experiments., , and , as evaluated by Student’s -test.

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