Pegylated Recombinant Human Arginase 1 Induces Autophagy and Apoptosis via the ROS-Activated AKT/mTOR Pathway in Bladder Cancer Cells

Figure 4

Autophagy induction and AKT/mTOR pathway activation after BCT-100 exposure. (a) T24 cells were transfected with the mRFP-GFP-LC3 adenovirus for 24 h and treated with BCT-100 (20 mU/ml) for another 24 h. Representative pictures of fluorescent LC3 puncta were captured by an inverted fluorescent microscope. (b, c) BCT-100-treated T24 cells were harvested at different time points. LC3B, p62, Beclin-1, p-AKT, AKT, p-mTOR, mTOR, and β-actin were tested by Western blot. All data are presented as the of three independent experiments., , and , as evaluated by Student’s -test.

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