Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production

Figure 6

Wogonin improves the IL-10 production in MSCs via GSK3β/AKT pathway. MSCs were stimulated by Wogonin at a different dose (12.5, 25, and 50 μM) in the presence of LPS. After 24 h stimulation, western blot was performed. The cell extract probed with antibodies as indicated, and GAPDH as loading control. Relative protein expression was calculated, respectively. (a) The representatives of the protein expression of HIF-1α, IL-10 as well as GSK3β, AKT, and Stat3 and their phosphorylated forms (p-GSK3β, p-AKT, and p-Stat3). The effects of Wogonin on the expression of HIF-1α and IL-10. (b) The effects of Wogonin on GSK3β. (c) AKT (d) and Stat3 (e) signaling pathways. All the western blotting experiments were repeated three times. Values are . The statistical significance of difference between two means was calculated with two-way ANOVA followed by multiple comparisons with test and Bonferroni correction, .

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