Research Article

Polycyclic Aromatic Hydrocarbons from Fine Particulate Matter Induce Oxidative Stress and the Inflammatory Response in Human Vocal Fold Fibroblast Cells

Figure 2

PM-induced ROS generation in hVFFs. (a) After treatment with 25 μg/mL PM for 24 h, the intracellular ROS levels were determined using the DCFH-DA probe by inverted fluorescence microscopy (magnification, 200x). Green color indicates DCF-positive cells. (b) Relative intensity of DCF fluorescence. All experiments were performed in triplicate. Values are the . compared to the control.
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