Polycyclic Aromatic Hydrocarbons from Fine Particulate Matter Induce Oxidative Stress and the Inflammatory Response in Human Vocal Fold Fibroblast Cells

<div>AhR and CYP1A1 knockdown inhibited PM-induced lipid peroxidation and oxidative DNA damage in hVFFs transfected with either AhR or CYP1A1 siRNA before treatment for 24 h with PM (25 <i>μ</i>g/mL). (a) Immunofluorescence staining of hVFFs to analyze 4-HNE levels as a marker of lipid peroxidation; signals were detected in a peroxidase reaction (red), nuclei were counterstained with DAPI (magnification, 200x). (b) Fluorescence intensity derived from 4-HNE. (c) The level of 8-oxoG, a hallmark of oxidative DNA damage, was measured based on 8-OHdG detection (red); nuclei were counterstained with DAPI (magnification: ×200). (d) Fluorescence intensity derived from 8-OHdG. All experiments were performed in triplicate. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Polycyclic Aromatic Hydrocarbons from Fine Particulate Matter Induce Oxidative Stress and the Inflammatory Response in Human Vocal Fold Fibroblast Cells 

Figure 6 | Polycyclic Aromatic Hydrocarbons from Fine Particulate Matter Induce Oxidative Stress and the Inflammatory Response in Human Vocal Fold Fibroblast Cells 