Research Article
Polycyclic Aromatic Hydrocarbons from Fine Particulate Matter Induce Oxidative Stress and the Inflammatory Response in Human Vocal Fold Fibroblast Cells
Figure 7
Effects of AhR and CYP1A1 silencing on PM-induced inflammatory cytokines in hVFFs transfected with either AhR or CYP1A1 siRNA before treatment for 24 h with PM (25 μg/mL). (a, b) IL-6 and IL-8 mRNA expression was quantified by qRT-PCR normalized to the GAPDH housekeeping gene. (c, d) The protein levels of IL-6 and IL-8 were measured using ELISA. All experiments were performed in triplicate. Values are the . compared to the untreated control and compared to the PM-treated control.
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