Research Article

Polycyclic Aromatic Hydrocarbons from Fine Particulate Matter Induce Oxidative Stress and the Inflammatory Response in Human Vocal Fold Fibroblast Cells

Figure 8

Inhibition of PM-induced IL-6 and IL-8 production by NAC in hVFFs pretreated with or without NAC (1 mM) for 2 h before exposure to PM for 24 h. After incubation, the cells were collected to analyze IL-6 and IL-8 gene expression by qRT-PCR and analysis of cell supernatants for IL-6 and IL-8 protein expression by ELISA. (a, b) Effects of NAC on IL-6 and IL-8 mRNA expression. (c, d) Effects of NAC on IL-6 and IL-8 secretion. Results of three separate experiments are displayed in graphs and are expressed as the . compared to the untreated control and compared to PM without NAC.
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