Circulating Metabolomic Analysis following Cecal Ligation and Puncture in Young and Aged Mice Reveals Age-Associated Temporal Shifts in Nicotinamide and Histidine/Histamine Metabolic Pathways

Figure 2

Comparison of baseline metabolomic status between young and aged animals. (a) PLS-DA plot of the metabolomic data from retro-orbital bleed samples from both aged (red) and young (green) animals, demonstrating distinct grouping and suggesting a clear difference at the metabolome level between young and aged animals at baseline. (b) Schematic representation of the statistical analysis algorithm used to identify the top 100 metabolites that best differentiate between young and aged animals at baseline. (c) Distribution of metabolite families among the original 566 metabolites in the dataset compared to the top 100 metabolites capable of differentiating young from aged. (d) Complete heatmap representing the top 100 metabolites, organized according to metabolite family. (e) Metabolome pathway map demonstrating specific metabolic pathway enrichment among the top 100 metabolites compared to the original 566 in the dataset.

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