Circulating Metabolomic Analysis following Cecal Ligation and Puncture in Young and Aged Mice Reveals Age-Associated Temporal Shifts in Nicotinamide and Histidine/Histamine Metabolic Pathways

Figure 4

Identification of the metabolic pathways that drive the response to CLP in aged animals. PCA plots of the aged sham (a) and CLP (b) metabolomes. Sham-treated animals demonstrated a slow return to baseline over time, though not to the same degree seen in young animals. On the other hand, aged CLP-treated animals experienced progressive derangement of the metabolome over time. (c) Profile of the top 100 metabolites that differentiate between CLP and sham interventions in aged mice over time. (d) Pathway analysis comparing enrichment of the top 100 metabolites compared to the 566 metabolites originally contained in the dataset. (e) Heatmap analysis demonstrating group average values of the top 100 metabolites at all CLP and sham surgical timepoints in aged animals, illustrating broad differences in metabolomic responses following CLP compared to sham intervention.

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