Dietary Beta-Hydroxy Beta-Methyl Butyrate Supplementation Alleviates Liver Injury in Lipopolysaccharide-Challenged Piglets

Table 1

Primers used for real-time quantitative PCR.

Genes	Forward (5-3)	Reverse (5-3)
HexoK 2	CTCATCACAACCGTTACCA	TGTCATTAGTGTCCTCATCC
L-PFK	CTGCACCGCATCATGGA	CCCCATCACCTCCAGAACA
PK	TCACTCCACAGACCTCAT	TACCTAGCCACCTGATGT
PDH	GCAGACTTACCGTTACCAT	GATAGCCGAGTTCTTCCAA
ACO	CTCGCAGACCCAGATGAAAT	TCCAAGCCTCGAAGATGAGT
L-CPT-1	GGACCGCCACCTGTTCTGCCTCTA	GCCCCCTCCGCTCGACACATAC
CS	TCTCAGCTCAGTGCAGCCATTACA	CTGCAACACAAGGTAGCTTTGCGA
ICDH β	TGTGGTTCCTGGTGAGAG	CGAGATTGAGATGCCGTAG
ICDH γ	GGTGGAGAGCCTCAAGAT	TGGTGGTGTTGTCTACGA
AMPKα1	AAATCGGCCACTACATCCTG	GGATGCCTGAAAAGCTTGAG
AMPKα2	AACATGGACGGGTTGAAGAG	CGCAGAAACTCACCATCTGA
Sirt1	CTGGAACAGGTTGCAGGAAT	CCTAGGACATCGAGGAACCA
PGC-1α	GATGTGTCGCCTTCTTGTTC	CATCCTTTGGGGTCTTTGAG
PCNA	TACGCTAAGGGCAGAAGATAATG	CTGAGATCTCGGCATATACGTG
Caspase-3	ACCCAAACTTTTCATAATTCA	ACCAGGTGCTGTAGAATATGC
TNF-α	TCCAATGGCAGAGTGGGTATG	AGCTGGTTGTCTTTCAGCTTCAC
COX2	ATGATCTACCCGCCTCACAC	AAAAGCAGCTCTGGGTCAAA
HSP70	GCCCTGAATCCGCAGAATA	TCCCCACGGTAGGAAACG
TLR4	TCAGTTCTCACCTTCCTCCTG	GTTCATTCCTCACCCAGTCTTC
MyD88	GATGGTAGCGGTTGTCTCTGAT	GATGCTGGGGAACTCTTTCTTC
IRAK1	CAAGGCAGGTCAGGTTTCGT	TTCGTGGGGCGTGTAGTGT
TRAF6	CAAGAGAATACCCAGTCGCACA	ATCCGAGACAAAGGGGAAGAA
NOD1	CTGTCGTCAACACCGATCCA	CCAGTTGGTGACGCAGCTT
NOD2	GAGCGCATCCTCTTAACTTTCG	ACGCTCGTGATCCGTGAAC
RIPK2	CAGTGTCCAGTAAATCGCAGTTG	CAGGCTTCCGTCATCTGGTT
NF-κB p65	AGTACCCTGAGGCTATAACTCGC	TCCGCAATGGAGGAGAAGTC
β-Actin	TGCGGGACATCAAGGAGAAG	AGTTGAAGGTGGTCTCGTGG

ACO: acyl-coenzyme A oxidase; AMPKα: AMP-activated protein kinase α; CS: citrate synthase; Hexok 2: hexokinase2; HSP70: heat shock protein 70; ICDH β: isocitrate dehydrogenase β; ICDH γ: isocitrate dehydrogenase γ; IRAK1: IL-1 receptor-associated kinase 1; L-CPT-1: liver carnitine palmitoyltransferase I; L-PFK: 6-phosphofructokinase (liver type-like); MyD88: myeloid differentiation factor 88; NODs: nucleotide-binding oligomerization domain protein; PCNA: proliferation cell nuclear antigen; PDH: pyruvate dehydrogenase; PGC-1α: peroxisome proliferator-activated receptor-g coactivator-1α; PK: pyruvate kinase; RIPK2: receptor-interacting serine/threonine-protein kinase 2; SIRT1: silent information regulator transcript 1; TLR4: toll-like receptor 4; TNF-α: tumor necrosis factor-α; TRAF6: TNF-α receptor-associated factor 6.