SM22α Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B

Figure 4

ZFP36 mediates circRasGEF1B-induced apoptotic programming of VSMCs. (a) The relative fold change of the mRNA of Ada, Tnfrsf-26, Relt, Mif, Cd74, Nradd, Ticam1, Cd5, Zfp36, and Zfp36l1 in MOVAS cells transfected with the pLCDH-circRasGEF1B plasmid compared to the pLCDH empty vector group. (b) Western blot and densitometric analysis of the expression of ZFP36 in MOVAS cells transfected with the pLCDH empty vector or pLCDH-circRasGEF1B plasmid. (c) qRT-PCR of remaining ZFP36 mRNA in pLCDH empty vector- or pLCDH-circRasGEF1B plasmid-transfected MOVAS cells stimulated by ActD stimulation for indicated time. Values represent from 3 independent experiments; , vs. empty vector group at the same time point. (d) qRT-PCR of ZFP36 mRNA in MOVAS cells transfected with si-Con or si-ZFP36. (e, f) TUNEL assay (e) or Western blot (f) for cell apoptosis in circRasGEF1B-overexpressed MOVAS cells transfected with si-Con or si-ZFP36. Data are presented as of three independent experiments. , , and .

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