SM22α Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B

Figure 5

circRasGEF1B guides ZFP36 to preferentially bind to and decay Bcl-2 mRNA in VSMCs. (a) qRT-PCR of Bcl-2 mRNA levels in circRasGEF1B-overexpressed MOVAS cells transfected with si-Con or si-ZFP36. (b) RIP assay of Bcl-2 mRNAs retrieved by using a ZFP36 antibody in MOVAS cells transfected with the pLCDH empty vector or pLCDH-circRasGEF1B plasmid. (c) RNA pull-down assay of ZFP36 protein retrieved by Bcl-2 mRNA probes in MOVAS cells transfected with the pLCDH empty vector or pLCDH-circRasGEF1B plasmid. (d) RIP assay of ZFP36 mRNAs retrieved by using a ZFP36 antibody in MOVAS cells transfected with the pLCDH empty vector or pLCDH-circRasGEF1B plasmid. (e) RNA pull-down assay of ZFP36 proteins retrieved by using ZFP36 mRNA probes in MOVAS cells transfected with the pLCDH empty vector or pLCDH-circRasGEF1B plasmid. (f) RIP assay of circRasGEF1B levels retrieved by using an IgG or ZFP36 antibody in MOVAS cells transfected with the pLCDH-circRasGEF1B plasmid. (g) RNA pull-down assay of ZFP36 proteins retrieved by using a circRasGEF1B probe in MOVAS cells transfected with the pLCDH-circRasGEF1B plasmid. (h) RNA pull-down assay of circRasGEF1B levels retrieved by using Bcl-2 or ZFP36 mRNA probes in MOVAS cells transfected with the pLCDH-circRasGEF1B plasmid. Data are presented as of three independent experiments. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)