SM22α Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B

Figure 6

circRasGEF1B directly interacts with both ZFP36 and Bcl-2 mRNAs in a sequence-specific manner. (a) RNA pull-down assay of ZFP36 proteins retrieved by using probes of circRasGEF1B deletion mutants in MOVAS cells transfected with circRasGEF1B deletion mutants. (b, c) RNA pull-down assay of ZFP36 proteins retrieved by using circRasGEF1B probes in MOVAS cells transfected with circRasGEF1B, block oligo 244-302 (b), or 244-302 deletion mutant (c). (d) RIP assay of circRasGEF1B retrieved by using a ZFP36 antibody in MOVAS cells transfected with circRasGEF1B, block oligo 244-302, or 244-302 deletion mutant. (e) RNA pull-down assay of Bcl-2 mRNA retrieved by using probes of circRasGEF1B deletion mutants in MOVAS cells transfected with circRasGEF1B deletion mutants. (f) RIP assay of Bcl-2 mRNA retrieved by using a ZFP36 antibody in MOVAS cells transfected with circRasGEF1B or deletion mutants. (g, h) qRT-PCR of Bcl-2 mRNA levels (g) or TUNEL assay of cell apoptosis (h) in MOVAS cells transfected with circRasGEF1B or deletion mutants. Bars: 100 μm (h). Data are presented as of three independent experiments. , , and .

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