Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway

Figure 2

PHLPP2 is downregulated and contributes to pyroptosis in UC. (a–d) Compared with the control group, the UC group exhibited significant weight loss, increased DAI, and shortened colon length. (e) H&E staining of colon sections showed aggravated inflammatory infiltration in the UC group (100x). (f) Coimmunofluorescence staining of PHLPP2 and E-cadherin or Iba1 showed that PHLPP2 was located in IECs and downregulated in the UC group. (g, h) The UC group had significantly enhanced IL-18 and IL-1β levels. (i) The levels of caspase-1 P20 and Gasdermin N were significantly elevated in the colons of the UC group. (j) LDH activity was significantly increased in the colons of the UC group. (k) PHLPP2 was significantly decreased in the colons of the UC patients.

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