Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway

Figure 3

PHLPP2 depletion exacerbates the DSS-induced colitis. (a–d) Compared with the WT group, the PHLPP2^-/- group exhibited significant weight loss, increased DAI, and shortened colon length after DSS administration. (e, f) The PHLPP2^-/- group had significantly enhanced IL-18 and IL-1β levels compared with the WT group after DSS administration. (g) H&E staining of colon sections showed aggravated inflammatory infiltration in the PHLPP2^-/- group (100x). (h) PHLPP2 was significantly decreased; however, the expressions of p-Akt, IKK, NF-κB p65, p-NF-κB p65, caspase-1 P20, and Gasdermin N dramatically increased in the PHLPP2^-/- group compared with the WT group after DSS administration. (i) LDH activity was significantly upregulated in the colons of the PHLPP2^-/- group compared with the WT group after DSS administration.

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