Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway

Figure 4

PHLPP depletion contributes to colonic organoid pyroptosis after LPS exposure. (a) Colonic organoids were cultured for 4 days in the WT group and PHLPP2^-/- group. (b) PHLPP2 was significantly decreased in the PHLPP2^-/- group. (c, d) The PHLPP2^-/- group had significantly enhanced IL-18 and IL-1β levels after LPS exposure. (e) The PHLPP2^-/- group had significantly increased the expressions of p-Akt, IKK, NF-κB p65, p-NF-κB p65, caspase-1 P20, and Gasdermin N after LPS exposure. (f) LDH activity was significantly upregulated in the colons of the PHLPP2^-/- group after LPS was induced. (g–i) The NF-κB signaling inhibitor can alleviate the inflammation and significantly decrease IL-18, IL-1β, and LDH levels in the PHLPP2^-/- group. (j) The NF-κB signaling inhibitor can significantly decrease the expression of caspase-1 P20 and Gasdermin N expression in the PHLPP2^-/- group.

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