Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway

Figure 5

PHLPP depletion contributes to FHC pyroptosis after LPS exposure. (a) The PHLPP2 was significantly decreased in the PHLPP2-siRNA group. (b, c) Compared with the control group, the PHLPP2-siRNA group had significantly enhanced IL-18 and IL-1β levels after LPS exposure. (d) The PHLPP2-siRNA group had significantly increased the expression of p-Akt, IKK, NF-κB p65, p-NF-κB p65, caspase-1 P20, and Gasdermin N after LPS exposure. (e) LDH activity was significantly increased in the colons of the PHLPP2-siRNA group after LPS exposure. (f) Pyroptosis was detected using scanning electron microscopy (SEM). Typical membrane integrity loss was found in the PHLPP2-siRNA group. (g–i) The NF-κB signaling inhibitor can alleviate the inflammation and significantly decrease IL-18, IL-1β, and LDH levels in the PHLPP2-siRNA group. (j) The NF-κB signaling inhibitor can significantly decrease caspase-1 P20 and Gasdermin N expressions in the PHLPP2-siRNA group.

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