Artemisinin Reverses Glucocorticoid-Induced Injury in Bone Marrow-Derived Mesenchymal Stem Cells through Regulation of ERK1/2-CREB Signaling Pathway

Figure 11

Protection of artemisinin was attenuated by siCREB. (a) BMSCs were transfected with 40 nM siCREB for 48 h, and the expression of T-CREB and GAPDH was detected by Western blot (). (b) Quantitative analysis of CREB relative expression using the ImageJ software (). versus control group was considered significantly different. BMSCs were transfected with 40 nM siCREB as above and pretreated with 1.0 μM artemisinin for 1 h followed by incubation with 30 μM DEXA for another 24 h. (c) Analysis of cell viability using MTT assay (). and were considered significantly different. (d) Analysis of apoptosis by FACS using Annexin V-FITC/PI staining (). (e) Quantitative analysis of (d). (f) Assessment of Δψm using JC-1 assay (). (g) Quantitative analysis of (f) (). , , and were considered significantly different. CTRL: control; Si-NC: Si-negative control; ART: artemisinin; DEXA: dexamethasone; ART+DEXA: treated with artemisinin followed by exposure to dexamethasone: siCREB + ART + DEXA, cells were transfected with siCREB, then pretreated with artemisinin followed by incubation with dexamethasone.

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