GPA Peptide Attenuates Sepsis-Induced Acute Lung Injury in Mice via Inhibiting Oxidative Stress and Pyroptosis of Alveolar Macrophage

Figure 6

GPA peptide ameliorated pyroptosis and oxidative stress in vitro. (a) The cytotoxicity of J774.A1 cells was determined using an LDH assay after they were exposed to various GPA concentrations for 6 hours. (b) J774.A1 cells were subjected to pretreatment with various doses of GPA for 6 hours before being exposed to LPS (1 μg/mL) for 3 hours, and cytotoxicity was measured using the LDH assay. (c, d) The proportion of pyroptotic cells (caspase-1⁺PI⁺) was detected using flow cytometry (FCM) after 1 h. (e) Commercial reactive oxygen species assay kits were used to assess the extracellular ROS levels. (f) Immunoblot analysis of the pore-forming mediator of pyroptosis. GSDMD cleavage was suppressed in J774.A1 cells following the LPS challenge for 6 hours. Data are shown as the . , , and vs. the control cohort; ^#, ^##, and ^### vs. the LPS cohort.

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