GPA Peptide Attenuates Sepsis-Induced Acute Lung Injury in Mice via Inhibiting Oxidative Stress and Pyroptosis of Alveolar Macrophage

Figure 8

GPA peptide blocked caspase-1 activation by inhibiting ROS production. J774.A1 cells were subjected to pretreatment with 5 mM NAC for 1 hour and subsequently subjected to LPS. (a) The CCK8 assay was used to determine cell viability in the presence or absence of NAC treatment. (b) The generation of ROS was monitored, and the buildup of ROS was quantified and displayed using a bar graph. (c) Caspase-1 activity was detected. J774.A1 cells were primed with LPS for 4 hours, then treated with GPA for 6 hours before being stimulated with H2O2 for 4 hours. Levels of the ROS (d) and caspase-1 activity (e) were detected in J774.A1 cells. Data are shown as the . , and vs. the control cohort; ^#, and ^## vs. the LPS cohort.

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