GPX4-Regulated Ferroptosis Mediates S100-Induced Experimental Autoimmune Hepatitis Associated with the Nrf2/HO-1 Signaling Pathway

Figure 1

Ferroptosis plays an important role in S100-induced autoimmune hepatitis. (a) Experimental protocol for the modeling of S100-induced AIH model mice. (b)–(d) The serum ALT, AST, and IgG expression levels in the control and AIH groups. (e) Representative H&E staining of liver tissue sections. The black arrow indicates lymphocytic infiltration (original magnification 20×). (f) TNF-α, IFNγ, IL-17, IL-10, and TGF-β levels in liver. (g) IHC staining of COX2 and GPX4 in liver sections (original magnification 20×). (h) Semiquantitative IHC results. (i)–(j) Western blotting showing protein expression of COX2, ACSL4, GPX4, and FTH1 in the pre-experimental control and AIH groups. GAPDH was used as a loading control; , compared with the control group.

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