[Retracted] S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways

Figure 7

CSNO inhibited mitophagy and ER stress in vitro. (a) A7R5 cells were pretreated with CSNO (50 μmol/L) for 1 hour and incubated with 0.5 μmol/L AngII for an additional 24 hours. Protein expression was determined by immunoblotting using specific antibodies as indicated. Representative images of PINK1, Bip, CHOP, LC3B, and P62 expression. (c–g) Quantification of corresponding images. (b) A7R5 cells were pretreated with CSNO (50 μmol/L) for 1 hour and incubated with 25 ng/mL IL-6 for an additional 24 hours. Protein expression was determined by immunoblotting using specific antibodies as indicated. (h–l) Quantification of corresponding images. (m–r) Bip, CHOP, and XBP1 mRNA levels were determined by RT-qPCR. These data are calculated from three independent experiments and are displayed as . vs. controls, ^# vs. AngII or IL-6 treatment.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)

(r)