[Retracted] Stearoyl-CoA Desaturase 1 Potentiates Hypoxic plus Nutrient-Deprived Pancreatic Cancer Cell Ferroptosis Resistance

Figure 2

SCD1 expression is positively related to pancreatic cancer H/NS condition and progression. (a) TCGA database analysis of the association of SCD1 expression with overall survival rate and disease-free survival rate of patients. (b) Expression of SCD1 in GEO database (GSE16515). Pancreatic tumor tissues compared to pancreatic normal tissues. (c) Basal SCD1 protein expression levels in Patu8988, PANC1, BXPC3, and SW1990 cells cultured in normal or H/NS condition were detected by western blot. β-Tubulin expression was detected as a loading control. (d, e) Western blot and qRT-PCR analysis of protein and mRNA expression levels of SCD1 in PANC1 and Patu8988 cells cultured in normal, nutrient starvation, hypoxia, or H/NS condition. β-Tubulin expression was detected as a loading control for western blot. ACTB mRNA expression was detected as a loading control for qRT-PCR. (f) ELISA-based analysis of the MUFA concentration in PANC1 and Patu8988 cells under normal and H/NS conditions. Nor represents normal condition. NS represents for nutrient starvation condition. Hy represents for hypoxia condition. H/NS represents for hypoxia combination with nutrient starvation condition. Experiments were repeated three times and the data are expressed as the . . . . .

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