[Retracted] Stearoyl-CoA Desaturase 1 Potentiates Hypoxic plus Nutrient-Deprived Pancreatic Cancer Cell Ferroptosis Resistance

Figure 3

SCD1-mediated MUFA formation protects PDAC cells from ferroptosis under H/NS. (a, b) Western blot and qRT-PCR analysis of shRNA-mediated knockdown of SCD1 protein and mRNA expression levels in PANC1 and Patu8988 cells. β-Tubulin expression was detected as a loading control. ACTB mRNA expression was detected as a loading control for qRT-PCR. (c) Sytox green staining analysis of PANC1 (Ctrl shRNA), SCD1 knockdown PANC1 (SCD1 shRNA1 and SCD1 shRNA2) cancer cells treated with erastin (5 μM) under H/NS condition. (d) PANC1 (Ctrl shRNA), SCD1 knockdown PANC1 (SCD1 shRNA1 and SCD1 shRNA2), Patu8988 (Ctrl shRNA), and SCD1 knockdown Patu8988 (SCD1 shRNA1 and SCD1 shRNA2) cancer cells were treated with erastin (5 μM for PANC1 and 40 μM for Patu8988) with or without OA (80 μM) and Fer-1 (1 μM for PANC1 and 5 μM for Patu8988) under H/NS condition. Cell viability was accessed via CCK8 assay. (e) MDA assay analysis of PANC1 (Ctrl shRNA), SCD1 knockdown PANC1 (SCD1 shRNA1 and SCD1 shRNA2), Patu8988 (Ctrl shRNA), and SCD1 knockdown Patu8988 (SCD1 shRNA1 and SCD1 shRNA2) treated with erastin (5 μM for PANC1 and 40 μM for Patu8988) with or without OA (80 μM) under H/NS condition. (f) Human MNSFA ELISA Kit was used to detect the content of monounsaturated fatty acids (MUFAs) in PANC1 (Ctrl shRNA), SCD1 knockdown PANC1 (SCD1 shRNA1 and SCD1 shRNA2), Patu8988 (Ctrl shRNA), and SCD1 knockdown Patu8988 (SCD1 shRNA1 and SCD1 shRNA2) cancer cells under normal or H/NS condition. (f) Analysis of the TCGA database for the correlation SCD1 mRNA expression level with the ferroptosis (ACSL3, SLC7A11, NRF2, and NQO1). The results are presented by heat map: . OA represents for oleic acid. Fer-1 represents for ferrostatin-1. Experiments were repeated three times and the data are expressed as the . . . . .

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