Effect of Methylene Blue on White Matter Injury after Ischemic Stroke

Figure 2

The protocol of Experiment 2, TTC staining, and sensorimotor assessment. (a) The protocol of Experiment 2. (b) Representative samples of TTC-stained brain sections. No ischemic lesion was found in the Sham group. Severe infarction was shown in MCAO mice. The white areas represented the infarction regions in these sections. MB reduced the infarction volume. MB administration with two doses is more effective than one dose. (c) Statistical analysis showed that MB administration at 24 h after MCAO with both 1 dose and 2 doses showed significantly decreased infarct volume compared with the MCAO group. (d) Foot fault test showed that rats in the MCAO group had higher foot fault rate. Meanwhile, rats in the MCAO+MB 1 dose group and the MCAO+MB 2 doses group showed significant decreased foot fault rate compared with those in the MCAO group. (e) Garcia neurofunctional score in the MCAO group, MCAO+MB 1 dose group, and MCAO+MB 2 doses group showed significant decrease compared with that in the Sham group. There was a tread of increase of the scores between the MCAO+MB 1 dose group and MCAO group, but the difference was not significant. Compared with that of the MCAO group, the score of MCAO+MB 2 doses group increased, showing a statistically significant difference. ; .

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