Effect of Methylene Blue on White Matter Injury after Ischemic Stroke

Figure 4

Black-gold staining of cortex and basal ganglia 24 h after ischemia-reperfusion. Black-gold staining of cortical myelin sheath and neurons in the Sham, MCAO, and MCAO+MB groups is shown in images A, B, and C, respectively. A2–C2 were the higher magnifications of the area outlined in A1–C1. Myelin sheath and neurons in basal ganglia in the Sham, MCAO, and MCAO+MB groups are shown in images D, E, and F, respectively. D2–F2 were the higher magnifications of the area outlined in D1–F1. Intact neurons and myelin sheath were observed, and Nissl body could clearly be seen in the Sham group. The MCAO injury destroyed the integrity, accompanied by reduced Nissl body and disordered myelin arrangement. However, the Nissl body in the MCAO+MB group was slightly increased, and the myelin sheath is relatively intact. Scale bar: 250 μm.