The Regulation of ROS- and BECN1-Mediated Autophagy by Human Telomerase Reverse Transcriptase in Glioblastoma

Figure 1

Bioinformatics analysis of hTERT in GBM and the influence of hTERT on telomerase activity, cell growth, and proliferation. (a) Box plot of LGG (low-grade glioma), GBM (glioblastoma) patients, and the corresponding normal controls from the GEPIA dataset. (b) Box plot of TERT gene expression in samples of Chinese glioma patients from CGGA dataset (above: CGGA mRNA-seq325: , , and ; below: CGGA mRNA-seq693: , , and ). (c) Survival analysis of GBM patients with high TERT levels and low TERT levels from TCGA dataset (TERT low: ; TERT high: ). (d) TERT mRNA levels and (e) protein levels in TERT knockdown cells and control cells. (f), (g) Telomerase activity assayed by TRAP. (h) Cell survival rate by clone formation experiment. (i) Growth curve of TERT knockdown cells and control cells by CCK-8. and were considered statistically significant.

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