Senkyunolide I Protects against Sepsis-Associated Encephalopathy by Attenuating Sleep Deprivation in a Murine Model of Cecal Ligation and Puncture

Figure 7

Improvement of cognitive dysfunction by Senkyunolide I of septic mice was reversed by sleep deprivation. Sleep deprivation was induced for 24 h after surgery. (a, b) Expression levels of BDNF and c-FOS were detected by western blot. (c–f) The relative quantification of BDNF/β-actin and c-FOS/β-actin in both hippocampus and cerebral cortex was analyzed. The levels of TNF-α (g), IL-1β (h), and IL-6 (i) in the serum were analyzed by ELISA. Freezing to context (j) and freezing to tone (k) were tested at 24 h after surgery, respectively. Values were presented as the (-8 for each group). vs. sham groups, vs. sham groups, and vs. sham groups; # vs. CLP+DMSO groups, ^## vs. CLP+DMSO groups; ^& vs. CLP+SEI group, ^&& vs. CLP+SEI group. SEI: Senkyunolide I; CLP: cecal ligation and puncture.

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