In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose-Derived Stem Cells

<div>Effect of photobiomodulation on FOXO1 levels in normal, wounded, diabetic, and diabetic wounded ADSCs irradiated at a wavelength of 660 and 830 nm with a fluence of 5 J/cm<sup>2</sup>. FOXO1 is stained green with FITC, and nuclei are stained blue with DAPI. Both the irradiated and nonirradiated groups displayed positive staining for FOXO1 proteins. Irradiated cells expressed less FOXO1 compared to the nonirradiated groups. In the nonirradiated groups, there was an increase in nuclear translocation of FOXO1 in diabetic and diabetic wounded models compared to their normal counterparts. This is due to the increase in oxidative stress induced by growing cells under hyperglycemic conditions. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose-Derived Stem Cells 

Figure 5 | In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose-Derived Stem Cells 