Research Article
In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose-Derived Stem Cells
Figure 6
Effect of photobiomodulation on AKT and FOXO1 in normal (N), wounded (W), diabetic (D), and diabetic wounded (DW) ADSCs irradiated at a wavelength of 660 and 830 nm with a fluence of 5 J/cm2. GAPDH was used as a loading control. L1: normal (0 J/cm2); L2: wounded (0 J/cm2); L3: diabetic (0 J/cm2); L4: diabetic wounded (0 J/cm2); L5: normal (5 J/cm2); L6: wounded (5 J/cm2); L7: diabetic (5 J/cm2); and L8: diabetic wounded (5 J/cm2). (a) Representative blots. (b, c) Quantification of the ratio of the intensity of target protein divided by the loading control protein. Irradiated N, W, D, and DW cells showed a significant increase in AKT levels (b) and decreased FOXO1 levels (c) as compared to the nonirradiated cells. Data is expressed as the (). Significant probabilities compared to the nonirradiated groups are shown as , , and .
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