Ligase Pellino3 Regulates Macrophage Action and Survival in Response to VSV Infection in RIG-I-Dependent Path

Figure 1

Lack of Pellino3 results in VSV-induced disintegration of BMDMs. (a) WT BMDMs were infected with VSV at MOI 1.5. Cell supernatants were collected 8 and 16 h of postinfection. Type I IFN was measured by bioassay; level of IP-10 and TNF was measured by ELISA as described under Materials and Methods. (b, c) WT and Peli3^-/- BMDMs were infected with VSV at MOI 1.5 and 0.015. Cell viability was evaluated by alamarBlue test 48 h of postinfection. Photographs were taken using 10x microscope objective. Results are representative of at least three independent experiments performed in triplicate (). Statistical analysis was carried out using the unpaired Student -test using GraphPad Prism 7.04. values of less than or equal to 0.05 were considered to indicate a statistically significant difference (, , and ).

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