PAR2 Deficiency Induces Mitochondrial ROS Generation and Dysfunctions, Leading to the Inhibition of Adipocyte Differentiation

<div>PAR2 regulated mitochondrial ROS and calcium levels in preadipocytes. Preadipocytes were isolated from sWAT of male WT mice (aged 5 weeks) and treated with the PAR2 agonist (SLIGRL-NH<sub>2</sub>) or antagonist (GB83). Mitochondrial ROS levels were observed using mitochondrial superoxide indicator MitoSOX by (a) fluorescent microscopy and (b) histogram of flow cytometry. (c) The data from flow cytometry were quantified. To observe mitochondrial calcium, Rhod-2, Ca<sup>2+</sup>-sensitive dye Rhod-2 to monitor changes in mitochondrial Ca<sup>2+</sup> was used for (d) flow cytometry. (e) The data from flow cytometry were quantified. 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Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: PAR2 Deficiency Induces Mitochondrial ROS Generation and Dysfunctions, Leading to the Inhibition of Adipocyte Differentiation 