Zi Shen Decoction Inhibits Growth and Metastasis of Lung Cancer via Regulating the AKT/GSK-3β/β-Catenin Pathway

Figure 3

ZSD inhibited the proliferation of lung cancer cells in vitro. (a) Calculated IC50 of different cancer cells and normal human BEAS-2B cells. The cells were treated with various concentrations of ZSD (0.25, 0.5, 1, 2, and 4 mg/mL) for 48 h and IC50 was calculated. (b–e) ZSD inhibited the viability of BEAS-2B, H1299, H1975, A549 cells in a time and dose-dependent manner. BEAS-2B and lung cancer cells were treated with different concentrations of ZSD for 12 h, 24 h, and 48 h. The percentages of viable cells were determined using MTT assay. , , and vs. the control group. (f) 4 mg/mL of ZSD exhibited more prominent inhibitory effect on lung cancer cells than BEAS-2B cells. (g) ZSD reduced colony formation of lung cancer cells. Cells were treated with indicated concentrations of ZSD and cultured for 14 days to form colonies. Colonies were stained with crystal violet and then counted. (h) Quantitation analysis of colony formation numbers. , , and vs. the control group.

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