General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease

Figure 2

Reduction of GCN5L1 ameliorates renal tubulointerstitial injury and proteinuria in DKD mice. (a) Experimental design for STZ-induced diabetic GCN5L1 knockdown mice. (b, c) Western blotting analysis showing the expression of GCN5L1 in the kidneys from diabetic GCN5L1 knockdown mice. Data are presented as the . (d, e) Immunofluorescent staining and fluorescence intensity for GCN5L1 expression in kidney sections. . (f) Urine albumin-to-creatinine ratio (UACR) in the kidneys from diabetic GCN5L1 knockdown mice. Independent experiments were performed in triplicate. (g, h) Immunohistochemistry staining and relative staining intensity for GCN5L1 expression in kidney sections. . (i) Hematoxylin and eosin staining, Masson’s trichrome staining, and periodic acid-Schiff staining in the kidneys from diabetic GCN5L1 knockdown mice. . ; and .

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