General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease

Figure 3

Downregulation of GCN5L1 activates MnSOD to scavenge ROS by reducing MnSOD acetylation on lysine 68 in TECs treated with high glucose. (a, b) Immunofluorescent staining and western blotting analysis of GCN5L1 protein expression in TECs exposed to high glucose. . (c, d) Coimmunoprecipitation of MnSOD and GCN5L1 in TECs. (e) Interaction of MnSOD and GCN5L1 in TECs visualized by Duolink proximity ligation assay. (f, g) Western blotting analysis of the protein levels of MnSOD and Ac-MnSOD K68 in TECs exposed to high glucose following GCN5L1 knockdown. (h) MnSOD activity was assessed in TECs exposed to high glucose following GCN5L1 knockdown. Independent experiments were performed in triplicate. (i) Cellular ROS was detected by MitoSOX staining in GCN5L1 knockdown TECs treated with high glucose. . Independent experiments were performed in triplicate. , , and .

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