General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease

Figure 4

Suppression of GCN5L1 alleviates inflammation and EMT through the MnSOD/ROS pathway in TECs under high glucose. (a) Protein levels of NLRP3, caspase-1, IL18, and IL1beta were detected by western blotting in TECs exposed to high glucose following knockdown of GCN5L1. (b) Protein levels of NLRP3, caspase-1, IL18, and IL1beta were detected by western blotting in GCN5L1 overexpressed TECs treated with NAC. (c) Western blotting analysis of the protein levels of NLRP3, caspase-1, IL18, and IL1beta after cotransfected with MnSODK68-R mutant plasmid and GCN5L1 silencing siRNA. (d, e) Western blotting analysis and immunofluorescent staining for E-cadherin and α-SMA in TECs exposed to high glucose following knockdown of GCN5L1. . (f) Protein levels of E-cadherin and α-SMA were detected by western blotting in GCN5L1 overexpressed TECs treated with NAC. (g) Western blotting analysis of the protein levels of E-cadherin and α-SMA after cotransfected with MnSODK68-R mutant plasmid and GCN5L1 silencing siRNA. Independent experiments were performed in triplicate.

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