General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease

Figure 5

Downregulation of GCN5L1 protects the kidney from hyperglycemia-induced EMT and inflammation via the MnSOD/ROS pathway in vivo. (a) Immunohistochemistry staining for acetylation level of MnSODK68 in the kidneys from diabetic GCN5L1 knockdown mice. . (b) DHE fluorescence to detect ROS in the kidneys from diabetic GCN5L1 knockdown mice. . (c) Immunohistochemistry staining for E-cadherin, α-SMA, and NLRP3 expression in the kidneys of mice. . (d–h) Western blotting analysis showing the expressions of E-cad, α-SMA, and NLRP3 in the kidneys of mice. Data are presented as the . ; , , and .

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