Cyclic Mechanical Stretch Ameliorates the Degeneration of Nucleus Pulposus Cells through Promoting the ITGA2/PI3K/AKT Signaling Pathway

Table 2

Validation of mRNA expression genes in the PI3K-AKT signaling pathway using qRT-PCR on the same sample set of the microarray study.

Gene	Fold change^a (microarray)	Fold change (qRT-PCR)	Fold change (qRT-PCR replication)
ITGA2	17.05	30.12	26.59
FGF1	2.69	3.24	5.36
COL2A1	2.15	2.97	3.51
BCL2L11	3.04	2.31	2.83
MYC	-3.35	-3.59	-3.81
IL7R	-3.29	-4.29	-2.73

^aFold change represents the difference multiple of gene expression between the tension group and the control group.