PAK1 Silencing Attenuated Proinflammatory Macrophage Activation and Foam Cell Formation by Increasing PPARγ Expression

Figure 5

Upregulated PPARγ expression by PAK1 silencing. (a) mRNA levels of the representative regulators for macrophage polarization in BMDMs by PAK1 knockdown with IL-4 stimulation. . compared with AdshRNA group. (b) Protein level of PPARγ in BMDMs by PAK1 knockdown with IL-4 or LPS stimulation. . or ^# compared with control group. (c–f). RT-PCR (c, d) and Western blot (e, f) analysis of M2 and M1 marker gene expression in macrophage transfected with AdshRNA or AdshPAK1 and treated with LPS or IL-4, which then cultured with G3335 or control. . compared with AdshRNA group; ^# vs. AdshPAK1 group; ^† vs. AdshRNA with G3335 group. (g) Immunoblotting with PPARγ or PAK1antibody was performed on co-IP of PAK1 using PAK1 antibody. (h) Schematic diagram of the molecular mechanisms underlying PAK1-regulated macrophage polarization and foam cell. PPARγ: peroxisome proliferator-activated receptor γ.

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