The Combination of β-Asarone and Icariin Inhibits Amyloid-β and Reverses Cognitive Deficits by Promoting Mitophagy in Models of Alzheimer’s Disease

Figure 4

Combination drug inhibited cell damage and toxic protein deposition in Aβ1-42 PC12 cell model of AD. (a) PC12 cells were treated with 7 μM Aβ1-42 for 12 h; then, we cultured the model cells with icariin, β-asarone, combination drug, or donepezil for another 12 h. (b) Cell proliferation and cytotoxicity LDH were detected as the above groups. (c) APP, PS1, BACE1, and Syn levels in PC12 cells with WB. (d) Quantification of protein expression levels in cells. At least three experiments were repeated independently with similar results. Full scans of all the blots are in the Supplementary Note. (e) IF assay of APP, PS1, and Syn was detected after the treatment (scale bar 100 μm and magnification 200x). SABC-DyLight marker protein expression, DAPI marker cell nucleus, also with a merge paper. Scale bar 100 μm. (f) Quantification of protein expression levels.

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