A Robust Panel Based on Mitochondrial Localized Proteins for Prognostic Prediction of Lung Adenocarcinoma

Figure 8

The regulation of the key genes in mitoRiskscore gene panel in the glycolysis metabolism, proliferation, and invasion of LUAD cells. (a) The expression of five genes in bronchial epithelial cell line (BEAS-2B) and lung adenocarcinoma cell lines (A549 and PC-9). (b) Knockdown efficiency of VDAC1 siRNA in A549 cell line. (c) Representative image and analysis of ECAR measurement in VDAC1 knockdown and control A549 cells. (d) Protein expression levels of LDHA, COXIV, SDHB, PGK1, and HKII in VDAC1 knockdown cells. (e) CCK-8 assays were used to evaluate A549 cell proliferation after VDAC1 knockdown. (f) GSEA analysis between patients with high and low expression of VDAC1. (g) The wound healing assay showed the migration ability of A549 cells after VADC1 knockdown. (h) Transwell experiments were performed to analyze the cell invasion ability after VDAC1 knockdown. , , , .

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