Vitamin B₁₂ Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway

Figure 4

VB12 regulates SIRT1 to inhibit oxidative stress by activating CBS. (a) Representative images showing Hoechst 33342 (blue) and PI (red) staining in pancreatic acinar cells treated with NaT, NaT+VB 12, or NaT+VB 12+EX-527 for 50 minutes, respectively. (b) The acinar cells were treated with NaT, NaT+VB 12, or NaT+VB 12+EX-527, respectively, and the protein levels of CBS, SIRT1, and PGC1-α were determined by Western blot analysis. (c–e) Relative band densities were quantified, and the normalized values are indicated in the histogram. (f, g) Representative images of ROS staining in acinar cells with DCFH-DA. (h–j) The protein expression changes of p-P38, P38, and Nrf2 in acinar cells treated with EX-527 were analyzed and quantified. vs. Con group, vs. Con group, vs. Con group, ^## vs. NaT group, ^# vs. NaT group, ^∆ vs. VB 12 (100 nM) group, ^∆∆ vs. VB 12 (100 nM) group, ^∆∆∆ vs. VB 12 (100 nM) group. Con: control group; NaT: acute pancreatitis group. All the experiments were repeated three times with similar results.

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