Vitamin B₁₂ Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway

Figure 5

VB 12 repairs mitochondrial function by activating the CBS/SIRT1 axis. (a, b) Representative image of in acinar cells stained with TMRM and quantification of the fluorescence density of . (c) ATP levels were measured by luminescence in pancreatic acinar cells; data were normalized to untreated control as 100%. (d) Western blot analysis showed the effect of VB 12 on SQSTM1(p62), LC3II, and Parkin protein levels in acinar cells. After incubating the cells with EX-527, the TMRM staining (e, f), ATP level (g), and the expression of SQSTM1 (p62), LC3II/LC3I, and Parkin protein (h) in each group were detected. vs. Con group, vs. Con group, ^# vs. NaT group, ^∆ vs. VB 12 (100 nM) group, ^∆∆ vs. VB 12 (100 nM) group. Con: control group; NaT: acute pancreatitis group. All the experiments were repeated three times with similar results.

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